Journal article
Dissecting the molecular evolution of fluoroquinolone-resistant Shigella sonnei
H Chung The, C Boinett, D Pham Thanh, C Jenkins, FX Weill, BP Howden, M Valcanis, N De Lappe, M Cormican, S Wangchuk, L Bodhidatta, CJ Mason, TNT Nguyen, T Ha Thanh, VP Voong, VT Duong, PHL Nguyen, P Turner, R Wick, PJ Ceyssens Show all
Nature Communications | Published : 2019
Abstract
Shigella sonnei increasingly dominates the international epidemiological landscape of shigellosis. Treatment options for S. sonnei are dwindling due to resistance to several key antimicrobials, including the fluoroquinolones. Here we analyse nearly 400 S. sonnei whole genome sequences from both endemic and non-endemic regions to delineate the evolutionary history of the recently emergent fluoroquinolone-resistant S. sonnei. We reaffirm that extant resistant organisms belong to a single clonal expansion event. Our results indicate that sequential accumulation of defining mutations (gyrA-S83L, parC-S80I, and gyrA-D87G) led to the emergence of the fluoroquinolone-resistant S. sonnei population ..
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Awarded by Wellcome Trust
Funding Acknowledgements
H.C.T. received a DPhil scholarship from the Tropical Network Fund, Nuffield Department of Medicine, University of Oxford. S.B. is a Sir Henry Dale Fellow, jointly funded by the Wellcome Trust and the Royal Society (100087/Z/12/Z). We thank I. Carle, M. Lejay-Collin, and C. Ruckly from the Institut Pasteur for their excellent technical assistance. F.X.W is funded by the Institut Pasteur, Sante Publique France, and by the French Government "Investissement d'Avenir" program (Integrative Biology of Emerging Infectious Diseases Laboratory of Excellence, grant no. ANR-10-LABX-62-IBEID).