Analysis of Epididymal Protein Synthesis and Secretion
Wei Zhou, Petra Sipila, Geoffry N De Iuliis, Matthew D Dun, Brett Nixon
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | JOURNAL OF VISUALIZED EXPERIMENTS | Published : 2018
The mammalian epididymis generates one of the most complex intraluminal fluids of any endocrine gland in order to support the post-testicular maturation and storage of spermatozoa. Such complexity arises due to the combined secretory and absorptive activity of the lining epithelial cells. Here, we describe the techniques for the analysis of epididymal protein synthesis and secretion by focusing on the model protein family of dynamin (DNM) mechanoenzymes; large GTPases that have the potential to regulate bi-directional membrane trafficking events. For the study of protein expression in epididymal tissue, we describe robust methodology for immunofluorescence labeling of target proteins in para..View full abstract
Awarded by National Health and Medical Research Council of Australia
The authors would like to acknowledge the National Health and Medical Research Council of Australia Project Grant APP1103176 for the support of this work.