IL-21 regulates SOCS1 expression in autoreactive CD8( ) T cells but is not required for acquisition of CTL activity in the islets of non-obese diabetic mice
Andrew PR Sutherland, Kate L Graham, Michelle Papadimitriou, Gaurang Jhala, Prerak Trivedi, Tara Catterall, Stacey Fynch, Thomas WH Kay, Helen E Thomas
Scientific Reports | NATURE RESEARCH | Published : 2019
In type 1 diabetes, maturation of activated autoreactive CD8+ T cells to fully armed effector cytotoxic T lymphocytes (CTL) occurs within the islet. At present the signals required for the maturation process are poorly defined. Cytokines could potentially provide the necessary "third signal" required to generate fully mature CTL capable of killing insulin-producing β-cells. To determine whether autoreactive CTL within islets respond to cytokines we generated non-obese diabetic (NOD) mice with a reporter for cytokine signalling. These mice express a reporter gene, hCD4, under the control of the endogenous regulatory elements for suppressor of cytokine signalling (SOCS)1, which is itself regul..View full abstract
Awarded by National Health and Medical Research Council of Australia (NHMRC)
This work was supported by a National Health and Medical Research Council of Australia (NHMRC) project grant (APP1071350), program grant (APP1037321) and fellowship (HET). APRS is supported by a Juvenile Diabetes Research Foundation (JDRF) Career Development Award and Diabetes Australia Millennium Award. St Vincent's Institute receives support from the Operational Infrastructure Support Scheme of the Government of Victoria.