Journal article

The Sub-picomolar Cu2 Dissociation Constant of Human Serum Albumin.

Karolina Bossak-Ahmad, Tomasz Frączyk, Wojciech Bal, Simon C Drew

ChemBioChem | Published : 2020


The apparent affinity of human serum albumin (HSA) for divalent copper has long been the subject of great interest, due to its presumed role as the major Cu2+ -binding ligand in blood and cerebrospinal fluid. Using a combination of electronic absorption, circular dichroism and room-temperature electron paramagnetic resonance spectroscopies, together with potentiometric titrations, we competed the tripeptide GGH against HSA to reveal a conditional binding constant of log c K Cu ..

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