The Mitochondrial Acyl-carrier Protein Interaction Network Highlights Important Roles for LYRM Family Members in Complex I and Mitoribosome Assembly
Marris G Dibley, Luke E Formosa, Baobei Lyu, Boris Reljic, Dylan McGann, Linden Muellner-Wong, Felix Kraus, Alice J Sharpe, David A Stroud, Michael T Ryan
MOLECULAR & CELLULAR PROTEOMICS | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2020
NDUFAB1 is the mitochondrial acyl carrier protein (ACP) essential for cell viability. Through its pantetheine-4'-phosphate post-translational modification, NDUFAB1 interacts with members of the leucine-tyrosine-arginine motif (LYRM) protein family. Although several LYRM proteins have been described to participate in a variety of defined processes, the functions of others remain either partially or entirely unknown. We profiled the interaction network of NDUFAB1 to reveal associations with 9 known LYRM proteins as well as more than 20 other proteins involved in mitochondrial respiratory chain complex and mitochondrial ribosome assembly. Subsequent knockout and interaction network studies in h..View full abstract
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Awarded by National Health and Medical Research Council (NHMRC)
M.G.D. is supported by the Australian Mitochondrial Disease Foundation and an Australian Government Research Training Program Scholarship. F.K. is supported by a Monash University graduate research fellowship. We acknowledge funding from the National Health and Medical Research Council (NHMRC Project Grants 1164459 to M.T.R., 1125390, 1140906 to M.T.R. and D.A.S.; NHMRC Fellowship 1140851 to D.A.S.). The authors declare that they have no conflicts of interest with the contents of this article.