Development of Plasmodium-specific liver-resident memory CD8( ) T cells after heat-killed sporozoite immunization in mice
Sonia Ghilas, Matthias H Enders, Rose May, Lauren E Holz, Daniel Fernandez-Ruiz, Anton Cozijnsen, Vanessa Mollard, Ian A Cockburn, Geoffrey McFadden, William R Heath, Lynette Beattie
European Journal of Immunology | WILEY | Published : 2021
Malaria remains a major cause of mortality in the world and an efficient vaccine is the best chance of reducing the disease burden. Vaccination strategies for the liver stage of disease that utilise injection of live radiation-attenuated sporozoites (RAS) confer sterile immunity, which is mediated by CD8+ memory T cells, with liver-resident memory T cells (TRM ) being particularly important. We have previously described a TCR transgenic mouse, termed PbT-I, where all CD8+ T cells recognize a specific peptide from Plasmodium. PbT-I form liver TRM cells upon RAS injection and are capable of protecting mice against challenge infection. Here, we utilize this transgenic system to examine whether ..View full abstract
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Awarded by Australian Research Council
Awarded by National Health and Medical Research Council (NHMRC)
We acknowledge the staff of the Biological Resources facility at The Peter Doherty Institute for Infection and Immunity for animal husbandry and the Melbourne Cytometry Platform for provision of flow cytometry services. This work was supported by the Australian Research Council (CE140100011), National Health and Medical Research Council (NHMRC; 1113293 and 1105817), and W.R.H is an NHMRC Senior Principal Research Fellow (1154457).