Conference Proceedings
A tractable preclinical model of optic nerve demyelination
Peter van Wijngaarden, Joseph P Paul, Vickie Hoi Ying Wong, Bang V Bui, Tobias D Merson
Investigative Ophthalmology and Visual Science | Association for Research in Vision and Ophthalmology | Published : 2019
Abstract
Purpose : Progress in the development of therapies to enhance remyelination in demyelinating diseases has been hampered by a lack of appropriate preclinical models - functional measures are often lacking or variable. We sought to develop a tractable and reproducible model of optic nerve demyelination with precise structural and functional measures. Methods : Oligodendrocytes of MBP-DTR 100a transgenic mice express diphtheria toxin receptor (DTR) and systemic diphtheria toxin (DT) administration induces diffuse demyelination of the central nervous system. In the present study we used retrobulbar DT injection to induce focal demyelination of the optic nerves of 3-month-old MBP-DTR 100a mice. ..
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Funding Acknowledgements
Support Multiple Sclerosis Research Australia Incubator Grant