Bacterial Load of Chlamydia trachomatis in the Posterior Oropharynx, Tonsillar Fossae, and Saliva among Men Who Have Sex with Men with Untreated Oropharyngeal Chlamydia

Tiffany R Phillips; Christopher K Fairley; Kate Maddaford; Jennifer Danielewski; Jane S Hocking; David Lee; Deborah A Williamson; Gerald Murray; Fabian Kong; Vesna De Petra; Catriona S Bradshaw; Marcus Y Chen; Rebecca Wigan; Anthony Snow; Benjamin P Howden; Suzanne M Garland; Eric PF Chow

Journal article

Bacterial Load of Chlamydia trachomatis in the Posterior Oropharynx, Tonsillar Fossae, and Saliva among Men Who Have Sex with Men with Untreated Oropharyngeal Chlamydia

Tiffany R Phillips, Christopher K Fairley, Kate Maddaford, Jennifer Danielewski, Jane S Hocking, David Lee, Deborah A Williamson, Gerald Murray, Fabian Kong, Vesna De Petra, Catriona S Bradshaw, Marcus Y Chen, Rebecca Wigan, Anthony Snow, Benjamin P Howden, Suzanne M Garland, Eric PF Chow

JOURNAL OF CLINICAL MICROBIOLOGY | AMER SOC MICROBIOLOGY | Published : 2020

DOI: 10.1128/JCM.01375-19

Abstract

The aim of this study was to determine whether Chlamydia trachomatis could be detected in saliva and if infection is specific to an anatomical site in the oropharynx. Men who have sex with men (MSM) who were diagnosed with oropharyngeal chlamydia at the Melbourne Sexual Health Centre in 2017-2018 were invited to participate upon returning for treatment. Swabs at the tonsillar fossae and posterior oropharynx and a saliva sample were collected. Throat samples were tested for C. trachomatis by the Aptima Combo 2 assay. The bacterial loads of C. trachomatis in all samples were assessed by quantitative PCR (qPCR) detecting the ompA gene. We calculated the positivity and bacterial load of C. trach..

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University of Melbourne Researchers

Ben Howden Author Microbiology and Immunology

Gerald Murray Author Obstetrics and Gynaecology Royal Women's Hospital/Mercy

Catriona Bradshaw Author Melbourne School of Population and Global Health

Jane Hocking Author Melbourne School of Population and Global Health

Suzanne Garland Wark Author Obstetrics and Gynaecology Royal Women's Hospital/Mercy

Related Projects (3)

Treatment efficacy of azithromycin 1g versus seven days doxycycline for the treatment of rectal chlamydia among men who have sex with men – a double-blind randomized controlled trial

Optimising treatment of gonorrhoea with Gepotidacin using pharmacokinetics

OPTIMISING THE TREATMENT OF SEXUALLY TRANSMITTED INFECTIONS (STIS) IN THE MOUTH AND RECTUM TO REDUCE ANTIMICROBIAL RESISTANCE AND BREAK ONGOING TRANSMISSION.

Grants

Awarded by National Health and Medical Research Council (NHMRC) program grant

Awarded by NHMRC Early Career Fellowship

Funding Acknowledgements

This work was funded by a National Health and Medical Research Council (NHMRC) program grant (GNT568971). E.P.F.C. was supported by an NHMRC Early Career Fellowship (GNT1091226).