Journal article

The role of cardiac transcription factor NKX2-5 in regulating the human cardiac miRNAome

Deevina Arasaratnam, Katrina M Bell, Choon Boon Sim, Kathy Koutsis, David J Anderson, Elizabeth L Qian, Edouard G Stanley, Andrew G Elefanty, Michael M Cheung, Alicia Oshlack, Anthony J White, Charbel Abi Khalil, James E Hudson, Enzo R Porrello, David A Elliott

Scientific Reports | NATURE PUBLISHING GROUP | Published : 2019

Abstract

MicroRNAs (miRNAs) are translational regulatory molecules with recognised roles in heart development and disease. Therefore, it is important to define the human miRNA expression profile in cardiac progenitors and early-differentiated cardiomyocytes and to determine whether critical cardiac transcription factors such as NKX2-5 regulate miRNA expression. We used an NKX2-5eGFP/w reporter line to isolate both cardiac committed mesoderm and cardiomyocytes. We identified 11 miRNAs that were differentially expressed in NKX2-5 -expressing cardiac mesoderm compared to non-cardiac mesoderm. Subsequent profiling revealed that the canonical myogenic miRNAs including MIR1-1, MIR133A1 and MIR208A were enr..

View full abstract

Grants

Awarded by NHMRC


Awarded by European Union's Seventh Framework Programme (FP7/2007-2013)


Awarded by Australian Research Council Strategic Initiative in Stem Cell Science


Awarded by Qatar National Research Foundation


Funding Acknowledgements

Funded by NHMRC project grant 1006304 (AW, DAE). DJA supported by the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement PIOF-GA-2010-276186. EGS, AGE and AO are Research Fellows of the NHMRC, E.R.P and JEH supported by National Heart Foundation fellowship. Research in the laboratories of DAE, EP, AGE and EGS was supported by the NHMRC, the Australian Research Council Strategic Initiative in Stem Cell Science (SR110001002), Stafford Fox Medical Research Foundation and The Royal Children's Hospital Foundation. DE, EGS and CAK receive funding from the Qatar National Research Foundation (NPRP9-169-3-024). Supported by the Victorian Government's Operational Infrastructure Support Program and Australian Government National Health and Medical Research Council (NHMRC) Independent Research Institute Infrastructure Support Scheme (IRIISS).