Electrical stimulation-induced osteogenesis of human adipose derived stem cells using a conductive graphene-cellulose scaffold
Jianfeng Li, Xiao Liu, Jeremy M Crook, Gordon G Wallace
Materials Science and Engineering C | ELSEVIER | Published : 2020
The versatile properties of graphene-based materials are enabling various tissue regeneration, towards meeting an ever increasing demand for replacement tissues due to injury through trauma and disease. In particular, an innate ability for graphene to promote osteogenic differentiation of stem cells, combined with the potential to enhance the biological activity of cells through electrical stimulation (ES) using graphene, supports its use for osteoinduction or reconstruction. In this paper, we describe a miniaturized graphene-cellulose (G-C) scaffold-based device that incorporates electroactive G-C 'paper' within a polystyrene chamber for concomitant cell culture and ES. The G-C electrodes p..View full abstract
Awarded by Australian Research Council (ARC) Centre of Excellence Scheme
Awarded by ARC
Awarded by ARC Industrial Transformation Training Centre in Additive Biomanufacturing
JL, XL, GGW and JMC conceived the study. JL executed the experiments. JL and JMC wrote the paper and all authors commented on the paper. JMC and GGW are co-senior corresponding authors. The authors wish to acknowledge funding from the Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012), equipment support by the Australian National Fabrication Facility (ANFF)Materials Node, and the use of facilities at the University of Wollongong Electron Microscopy Centre (EMC). GGW acknowledges the support of the ARC through an ARC Laureate Fellowship (FL110100196). GGW and XL acknowledge the support of the ARC Industrial Transformation Training Centre in Additive Biomanufacturing (IC160100026). JL would like to thank AINSE Ltd for providing financial assistance. The authors would also like to thank Assoc. Prof. Chee O. Too for his valuable comments on the manuscript, Dr Kerry Gilmore for technical support in cell related experiments, Dr Patricia Hayes for Raman measurements and Dr Dongqi Shi for support with XPS measurements.