Journal article

Identification and Mobilization of a Cryptic Antibiotic Biosynthesis Gene Locus from a Human-Pathogenic Nocardia Isolate

Marion Herisse, Keishi Ishida, Jessica L Porter, Howden Ben, Christian Hertweck, Timothy P Stinear, Sacha J Pidot

ACS Chemical Biology | AMER CHEMICAL SOC | Published : 2020

Abstract

The genus Nocardia contains >50 human pathogenic species that cause a range of illnesses from skin and soft tissue infections to lung and brain infections. However, despite their membership in the most prominent family of secondary metabolite producers (the Actinomycetes), the ability of Nocardia species, especially those that cause human infections, to produce secondary metabolites has not been as well studied. Using genome mining, we have investigated cryptic secondary metabolite biosynthesis gene clusters from Nocardia species and identified a conserved locus within human pathogenic strains of Nocardia brasiliensis and Nocardia vulneris. Direct capture and heterologous expression in a Str..

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Grants

Awarded by Australian National Health and Medical Research Council


Funding Acknowledgements

This work was supported by an Australian National Health and Medical Research Council grant (GNT1105522).