Journal article

The cation-dependent G-proteins: In a class of their own

Miriam-Rose Ash, Megan J Maher, J Mitchell Guss, Mika Jormakka



G-proteins are some of the most important and abundant enzymes, yet their intrinsic nucleotide hydrolysis reaction is notoriously slow and must be accelerated in vivo. Recent experiments on dynamin and GTPases involved in ribosome assembly have demonstrated that their hydrolysis activities are stimulated by potassium ions. This article presents the hypothesis that cation-mediated activation of G-proteins is more common than currently realised, and that such GTPases represent a structurally and functionally unique class of G-proteins. Based on sequence analysis we provide a list of predicted cation-dependent GTPases, which encompasses almost all members of the TEES, Obg-HflX, YqeH-like and dy..

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University of Melbourne Researchers


Awarded by National Health and Medical Research Council

Funding Acknowledgements

This study was supported by the National Health and Medical Research Council (No. 570784 and 632703). MJ is a National Health and Medical Research Council Career Development Fellow. M-RA was the recipient of an Australian Postgraduate Award during the course of this work.