Crystal structure of A3B3 complex of V-ATPase from Thermus thermophilus

Abstract

Vacuolar-type ATPases (V-ATPases) exist in various cellular membranes of many organisms to regulate physiological processes by controlling the acidic environment. Here, we have determined the crystal structure of the A 3 B 3 subcomplex of V-ATPase at 2.8 resolution. The overall construction of the A 3 B 3 subcomplex is significantly different from that of the α 3 Β 3 sub-domain in F o F 1-ATP synthase, because of the presence of a protruding bulge domain feature in the catalytic A subunits. The A 3 B 3 subcomplex structure provides the first molecular insight at the catalytic and non-catalytic interfaces, which was not possible in the structures of the separate subunits alone. Specifically, ..