Cyclic AMP in human preterm infant blood is associated with increased TLR-mediated production of acute-phase and anti-inflammatory cytokines in vitro
Tobias Strunk, Simon D van Haren, Julie Hibbert, Matthew Pettengill, Al Ozonoff, Jop Jans, Simone S Schueller, David Burgner, Ofer Levy, Andrew J Currie
Pediatric Research | SPRINGERNATURE | Published : 2020
BACKGROUND: Preterm infants are at high risk of infection and have distinct pathogen recognition responses. Suggested mechanisms include soluble mediators that enhance cellular levels of cAMP. The aim of this study was to assess the relationship between blood cAMP concentrations and TLR-mediated cytokine production in infants during the first month of life. METHODS: Cord and serial peripheral blood samples (days of life 1-28) were obtained from a cohort of very preterm (<30 weeks' gestational age) and term human infants. Whole-blood concentrations of cAMP and FSL-1 and LPS in vitro stimulated cytokine concentrations were measured by ELISA and multiplex bead assay. RESULTS: cAMP concentration..View full abstract
This project was supported by a National Health & Medical Research Council (NHMRC) of Australia project grant award (572548). S.S.S. was supported by a Max-Kade fellowship. D.B. is supported by an NHMRC Senior Research Fellowship (1046518). Research at the Murdoch Children's Research Institute is supported by the Victorian Government's Operational Infrastructure Program. Work in O.L.'s laboratory was supported by U.S. National Institutes of Health (NIH) National Institutes of Allergy and Infectious Diseases (NIAID) grants 1RO1AI00135-01, Human Immunology Project Consortium (HIPC) award 1U19AI118608-01A1 and U01AI12484-01, as well as Global Health (OPPGH5284) and Grand Challenges Explorations (OPP1035192) awards from the Bill & Melinda Gates Foundation. The Precision Vaccines Program is supported by an internal award from the Boston Children's Hospital Department of Pediatrics.