Journal article

Comparative spatial lipidomics analysis reveals cellular lipid remodelling in different developmental zones of barley roots in response to salinity

Lenin D Sarabia, Berin A Boughton, Thusitha Rupasinghe, Damien L Callahan, Camilla B Hill, Ute Roessner

Plant, Cell & Environment | WILEY | Published : 2019

Abstract

Salinity-induced metabolic, ionic and transcript modifications in plants have routinely been studied using whole plant tissues, which do not provide information on spatial tissue responses. The aim of this study was to assess the changes in the lipid profiles in a spatial manner and to quantify the changes in the elemental composition in roots of seedlings of four barley cultivars before and after a short-term salt stress. We used a combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS), inductively coupled plasma mass spectrometry (ICP-MS), matrix-assisted laser desorption/ionization (MALDI-MSI) and Reverse Transcription - quantitative real time polymerase chain reaction (..

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