Immunohistochemical classification of sensory and autonomic neurons projecting to the lower urinary tract in rats [keast-001] v1

Abstract

This collection describes the procedures required to label, visualise, characterise and quantify neurons that innervate the lower urinary tract tissues of adult male and female Sprague-Dawley rats. This collection includes protocols for: STAGE 1: Surgery to micro-inject fluorescent retrograde tracer dyes into one or more sites within the lower urinary tract STAGE 2: Intracardiac perfusion with fixative to preserve neural tissues of interest STAGE 3: Fluorescence immunohistochemistry of ganglion cryosections.