Journal article

Correspondence Between Behavioral, Physiological, and Anatomical Measurements of Visual Function in Inhibitory Neuron Ablated Zebrafish

Jiaheng Xie, Patrick T Goodbourn, Bang Bui, Tamar E Sztal, Patricia R Jusuf

Investigative Opthalmology & Visual Science | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2019


Purpose: To compare the effects of reduced inhibitory neuron function in the retina across behavioral, physiological, and anatomical levels. Methods: Inhibitory neurons were ablated in larval zebrafish retina. The Ptf1a gene, which determines inhibitory neuron fate in developing vertebrates, was used to express nitroreductase. By exposing larvae to the prodrug metronidazole, cytotoxicity was selectively induced in inhibitory neurons. Visual phenotypes were characterized at behavioral, physiological, and anatomical levels using an optomotor response (OMR) assay, electroretinography (ERG), and routine histology, respectively. Nonvisual locomotion was also assessed to reveal any general behavio..

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Awarded by Australian Research Council

Funding Acknowledgements

The authors thank the staff from the zebrafish facility at the Walter and Eliza Hall Institute of Medical Research for animal maintenance. Supported by a grant from the Melbourne Neuroscience Institute (to PTG, PRJ, BVB). PTG was supported by a Discovery Early Career Researcher Award from the Australian Research Council (DE160100125).