Does the apoptosis pathway play a critical role in gonocyte transformation?
Melissa Yixin Tien, Sanduni Amaya Abeydeera, Hyun-Jung Cho, Gulcan Sarila, Angelique Catubig, Emily Burton, John Hutson, Ruili Li
Journal of Pediatric Surgery | W B SAUNDERS CO-ELSEVIER INC | Published : 2020
PURPOSE: Undescended Testes (UDT) are prevalent in 2%-5% of male infants and cause malignancy and infertility. During germ cell development, abnormal gonocytes usually undergo apoptosis. This process is believed to involve BAX (Bcl-2 Associated X) protein in clearing abnormal gonocytes which may fail in UDT, resulting in persisting gonocytes causing seminomas later in life. We aim to investigate the role of BAX in gonocyte apoptosis. MATERIALS AND METHODS: BAXKO (BAX-knockout) mice were back-crossed to OG2 mice (Oct4-promoter driving enhanced green fluorescent protein-eGFP) to produce BAXOG2 mice. Testes (wildtype-BAX+/+, heterozygous-BAX+/- and homozygous-BAX-/- mice, n = 6/group) on postna..View full abstract
Awarded by Australian National Health and Medical Research Council
This study is supported by the Australian National Health and Medical Research Council (Grant Number APP1127109) and the Victorian Government's Operational Infrastructure Support Programme.