Journal article

A LAMP assay for the rapid and robust assessment of Wolbachia infection in Aedes aegypti under field and laboratory conditions

Moshe E Jasper, Qiong Yang, Perran A Ross, Nancy Endersby-Harshman, Nicholas Bell, Ary A Hoffmann

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2019

Abstract

With Wolbachia-based arbovirus control programs being scaled and operationalised around the world, cost effective and reliable detection of Wolbachia in field samples and laboratory stocks is essential for quality control. Here we validate a modified loop-mediated isothermal amplification (LAMP) assay for routine scoring of Wolbachia in mosquitoes from laboratory cultures and the field, applicable to any setting. We show that this assay is a rapid and robust method for highly sensitive and specific detection of wAlbB Wolbachia infection within Aedes aegypti under a variety of conditions. We test the quantitative nature of the assay by evaluating pooled mixtures of Wolbachia-infected and unin..

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Grants

Awarded by Wellcome Trust


Awarded by National Health and Medical Research Council (AU)


Funding Acknowledgements

AH received a grant (#108508) from the Wellcome Trust (https://wellcome.ac.uk/).AH received a grant (Program Grant #1132412) from the National Health and Medical Research Council (AU), (https://www.nhmrc.gov.au/).AH received a grant (Fellowship Grant #1118640) from the National Health and Medical Research Council (AU), (https://www.nhmrc.gov.au/).The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.