Assessing the Role of MIF in Plasmodium spp. Infections Using Ex Vivo Models
Elizabeth H Aitken, J Harris (ed.), EF Morand (ed.)
Methods in molecular biology (Clifton, N.J.) | HUMANA PRESS INC | Published : 2020
Ex vivo techniques are a valuable tool for the investigation of how immune cells respond to Plasmodium spp. antigen, allowing examination of various aspects of the immune response under controlled conditions. Here we describe how to isolate peripheral blood mononuclear cells (PBMC) from donors and coculture them with purified P. falciparum-infected red blood cells (iRBC) to investigate the role of MIF during Plasmodium spp. infection.