Journal article

Retooling phage display with electrohydrodynamic nanomixing and nanopore sequencing

Lyndon J Raftery, Christopher B Howard, Yadveer S Grewal, Ramanathan Vaidyanathan, Martina L Jones, Will Anderson, Darren Korbie, Tania Duarte, Duc Cao Minh, Hoang Nguyen Son, Lachlan JM Coin, Stephen M Mahler, Matt Trau

LAB ON A CHIP | ROYAL SOC CHEMISTRY | Published : 2019

Abstract

Phage display methodologies offer a versatile platform for the isolation of single-chain Fv (scFv) molecules which may be rebuilt into monoclonal antibodies. Herein, we report on a complete workflow termed PhageXpress, for rapid selection of single-chain Fv sequences by leveraging electrohydrodynamic-manipulation of a solution containing phage library particles to enhance target binding whilst minimizing non-specific interactions. Our PhageXpress technique is combined with Oxford Nanopore Technologies' MinION sequencer and custom bioinformatics to achieve high-throughput screening of phage libraries. We performed 4 rounds of biopanning against Dengue virus (DENV) non-structural protein 1 (NS..

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University of Melbourne Researchers

Grants

Awarded by National Breast Cancer Foundation of Australia


Funding Acknowledgements

Although not directly funding the research work in this paper, we acknowledge the funding received by our laboratory from the National Breast Cancer Foundation of Australia (CG-12-07). This grant has significantly contributed to the environment to stimulate the research described here.