Journal article
Skeletal muscle atrophy occurs slowly and selectively during prolonged aestivation in Cyclorana alboguttata (Günther 1867)
BL Mantle, NJ Hudson, GS Harper, RL Cramp, CE Franklin
Journal of Experimental Biology | COMPANY BIOLOGISTS LTD | Published : 2009
DOI: 10.1242/jeb.033688
Abstract
We investigated the effect of prolonged immobilisation of six and nine months duration on the morphology and antioxidant biochemistry of skeletal muscles in the amphibian aestivator Cyclorana alboguttata. We hypothesised that, in the event of atrophy occurring during aestivation, larger jumping muscles were more likely to be preserved over smaller non-jumping muscles. Whole muscle mass (g), muscle cross-sectional area (CSA) (μm2), water content (%) and myofibre number (per mm2) remained unchanged in the cruralis muscle after six to nine months of aestivation; however, myofibre area (μm2) was significantly reduced. Whole muscle mass, water content, myofibre number and myofibre CSA remained un..
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Awarded by AEC
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Funding Acknowledgements
This project was funded by an ARC Discovery Grant awarded to C. E. F. B. L. M. was supported by a University of Queensland Mid-Year Scholarship. The authors would like to thank Lina Daddow for technical assistance. The experimental procedures were in full compliance with AEC (# SIB/572/06/UQ) and EPA (# WISP03572406) regulations. B. L. M. (nee Symonds) developed project design, acquired and analysed data, drafted the manuscript. N.J.H. contributed to project design, provided assistance with the acquisition and interpretation of TAP data and critical revision of manuscript draft. G. S. H. provided access to equipment and facilities needed for acquisition of TAP data. R. L. C. provided technical support and revised the manuscript. C. E. F. contributed to project design, provided financial and technical assistance with data acquisition and interpretation, and provided critical revision of manuscript draft.