Journal article
Expression of developmentally important axon guidance cues in the adult optic chiasm
R Conceição, RS Evans, CS Pearson, B Hänzi, A Osborne, SS Deshpande, KR Martin, AC Barber
Investigative Ophthalmology and Visual Science | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2019
Abstract
PURPOSE. Regeneration of optic nerve axons after injury can be facilitated by several approaches, but misguidance at the optic chiasm is often observed. We characterized guidance cues in the embryonic visual system and adult optic chiasm before and after optic nerve crush (ONC) injury to better understand barriers to optic nerve regeneration in adults. METHODS. Radial glial (RC2/BLBP/Slit1), developmental (Pax2) and extracellular markers (CSPG: H2B/CS-56) were assessed in C57BL/6J mice by immunohistochemistry. RC2, BLBP, Slit1, and CSPG are known inhibitory guidance cues while Pax2 is a permissive guidance cue. RESULTS. At embryonic day 15.5 (E.15.5), RC2 and BLBP were identified superior to..
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Funding Acknowledgements
Supported by a Sir Henry Wellcome Post Doctoral Fellowship (ACB), the Cambridge Eye Trust, the Jukes Glaucoma Research Fund, Fight for Sight UK, King's College, and research in the laboratory is supported by core funding from Wellcome and MRC to the Wellcome-MRC Cambridge Stem Cell Institute.