Ultrasensitive and label-free biosensor for the detection of Plasmodium falciparum histidine-rich protein II in saliva.
Gita V Soraya, Chathurika D Abeyrathne, Christelle Buffet, Duc H Huynh, Shah Mukim Uddin, Jianxiong Chan, Efstratios Skafidas, Patrick Kwan, Stephen J Rogerson
Scientific Reports | Nature Publishing Group | Published : 2019
Malaria elimination is a global public health priority. To fulfil the demands of elimination diagnostics, we have developed an interdigitated electrode sensor platform targeting the Plasmodium falciparum Histidine Rich Protein 2 (PfHRP2) protein in saliva samples. A protocol for frequency-specific PfHRP2 detection in phosphate buffered saline was developed, yielding a sensitivity of 2.5 pg/mL based on change in impedance magnitude of the sensor. This protocol was adapted and optimized for use in saliva with a sensitivity of 25 pg/mL based on change in resistance. Further validation demonstrated detection in saliva spiked with PfHRP2 from clinical isolates in 8 of 11 samples. With a turnaroun..View full abstract
Awarded by Bill & Melinda Gates Foundation through the Grand Challenges Explorations Initiative
Awarded by National Health and Medical Research Council of Australia
This work was funded by the Bill & Melinda Gates Foundation through the Grand Challenges Explorations Initiative (OPP1151367) and by a Program Grant (1092789) from the National Health and Medical Research Council of Australia. The work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). E. S. thanks the generous support of Sue and Leigh Clifford in establishing the Chair in Neural Engineering. G.V.S. was supported by the Indonesian Endowment Fund for Education (LPDP). P.K. is supported by a Practitioner Fellowship from the National Health and Medical Research Council of Australia (APP1136427).