Two-photon imaging of dendritic calcium dynamics in vivo

Abstract

© Springer Science+Business Media, LLC, part of Springer Nature 2019. Developments in microscopes and indicators over the past decade have paved the way for recording neural dynamics in vivo. Previously inaccessible small neuronal processes, such as dendrites and axons, can now be probed in vivo using two-photon microscopy, revealing their important role in information processing during behaviour. To perform such experiments, various tools, techniques and considerations are required. In this chapter, the procedures for recording dendritic calcium dynamics in vivo are detailed in a step-by-step manner. The various sources of calcium contributing to the recorded transients are discussed, and d..