Journal article

Concise review: Human articular cartilage repair: Sources and detection of cytotoxicity and genotoxicity in photo-crosslinkable hydrogel bioscaffolds

Cheryl Lee, Cathal D O'Connell, Carmine Onofrillo, Peter FM Choong, Claudia Di Bella, Serena Duchi

STEM CELLS Translational Medicine | WILEY | Published : 2019

Abstract

Three-dimensional biofabrication using photo-crosslinkable hydrogel bioscaffolds has the potential to revolutionize the need for transplants and implants in joints, with articular cartilage being an early target tissue. However, to successfully translate these approaches to clinical practice, several barriers must be overcome. In particular, the photo-crosslinking process may impact on cell viability and DNA integrity, and consequently on chondrogenic differentiation. In this review, we primarily explore the specific sources of cellular cytotoxicity and genotoxicity inherent to the photo-crosslinking reaction, the methods to analyze cell death, cell metabolism, and DNA damage within the bios..

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Grants

Awarded by Sylvia and Charles Viertel Charitable Foundation


Funding Acknowledgements

St Vincent's Hospital (Melbourne) Research Endowment Fund; Sylvia and Charles Viertel Charitable Foundation, Grant/Award Number: VIERCI2018003; Victorian Medical Research Acceleration Fund