Journal article

Imaging and Quantification of Myelin Integrity After Injury With Spectral Confocal Reflectance Microscopy

David G Gonsalvez, SangWon Yoo, Jessica L Fletcher, Rhiannon J Wood, Georgina A Craig, Simon S Murray, Junhua Xiao



Developing a high-throughput approach to quantify the extent of myelin integrity in preclinical models of demyelinating diseases will enhance our capacity to identify novel therapies for myelin repair. In light of the technical limitations of electron microscopy and immunohistochemical analyses of myelination, we have utilized a novel imaging technique, spectral confocal reflectance (SCoRe) microscopy. SCoRe takes advantage of the optically reflective properties of compact myelin, allowing the integrity of compact myelin to be quantified over the course of the cuprizone-induced model of central demyelination. We applied SCoRe imaging on fixed frozen brain sections. SCoRe analysis of control ..

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Awarded by Australian Research Council

Awarded by Australian National Health and Medical Research Council (NHMRC)

Awarded by NHMRC ECF Fellowship

Awarded by Multiple Sclerosis Research Australia Postdoctoral Fellowship

Funding Acknowledgements

We would like to thank all grant sponsors: Australian Research Council Discovery Project Grant (#DP180102397) and the Australian National Health and Medical Research Council (NHMRC) Project Grant (#APP1058647) to JX; NHMRC ECF Fellowship (#APP1111041) to DG; Multiple Sclerosis Research Australia Postdoctoral Fellowship (#14-056) to JF; and the Sir John Eccles Vacation Scholarship and the Melbourne Research scholarship to SY and GC. Confocal imaging was performed at the Biological Optical Microscope Platform, The University of Melbourne.