HBO1 (KAT7) Does Not Have an Essential Role in Cell Proliferation, DNA Replication, or Histone 4 Acetylation in Human Cells
Andrew J Kueh, Samantha Eccles, Leonie Tang, Alexandra L Garnham, Rose E May, Marco J Herold, Gordon K Smyth, Anne K Voss, Tim Thomas
Molecular and Cellular Biology | American Society for Microbiology | Published : 2020
HBO1 (MYST2/KAT7) is essential for histone 3 lysine 14 acetylation (H3K14ac) but is dispensable for H4 acetylation and DNA replication in mouse tissues. In contrast, previous studies using small interfering RNA (siRNA) knockdown in human cell lines have suggested that HBO1 is essential for DNA replication. To determine if HBO1 has distinctly different roles in immortalized human cell lines and normal mouse cells, we performed siRNA knockdown of HBO1. In addition, we used CRISPR/Cas9 to generate 293T, MCF7, and HeLa cell lines lacking HBO1. Using both techniques, we show that HBO1 is essential for all H3K14ac in human cells and is unlikely to have a direct effect on H4 acetylation and only ha..View full abstract
Awarded by Australian National Health and Medical Research Council
This work was supported by the Australian National Health and Medical Research Council under senior research fellowships 1003435 (T.T.) and 575512 (A.K.V.) and project grants 1029481, 103070, 1051078 (to T.T. and A.K.V.), and 1049720 (to M.J.H.) and by operational infrastructure grants from the Australian Federal Government (IRISS) and the Victorian State Government (OIS).