Journal article

Transient MicroRNA Expression Enhances Myogenic Potential of Mouse Embryonic Stem Cells

Joanna Bem, Iwona Grabowska, Maciej Daniszewski, Dorota Zawada, Areta M Czerwinska, Lukasz Bugajski, Katarzyna Piwocka, Anna Fogtman, Maria A Ciemerych

Stem Cells | WILEY | Published : 2018

Abstract

MicroRNAs (miRNAs) are known regulators of various cellular processes, including pluripotency and differentiation of embryonic stem cells (ESCs). We analyzed differentiation of two ESC lines-D3 and B8, and observed significant differences in the expression of miRNAs and genes involved in pluripotency and differentiation. We also examined if transient miRNA overexpression could serve as a sufficient impulse modulating differentiation of mouse ESCs. ESCs were transfected with miRNA Mimics and differentiated in embryoid bodies and embryoid body outgrowths. miRNAs involved in differentiation of mesodermal lineages, such as miR145 and miR181, as well as miRNAs regulating myogenesis (MyomiRs)-miR1..

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University of Melbourne Researchers

Grants

Awarded by National Science Centre, Poland (Narodowe Centrum Nauki)


Funding Acknowledgements

We thank Katarzyna Janczyk-Ilach and Wladyslawa Streminska for providing fantastic technical and administrative support, Marta Koblowska for her comments on the manuscript and Radoslaw Wojtowicz (OpenExome) for his help. This work was supported by grant provided by budget funds from National Science Centre, Poland (Narodowe Centrum Nauki) for Maria A. Ciemerych-Litwinienko-2012/05/B/NZ1/00024 for 2013-2017.