Journal article

Human Immunodeficiency Virus (HIV)-Infected CCR6( ) Rectal CD4( ) T Cells and HIV Persistence On Antiretroviral Therapy

Jenny L Anderson, Gabriela Khoury, Remi Fromentin, Ajantha Solomon, Nicolas Chomont, Elizabeth Sinclair, Jeffrey M Milush, Wendy Hartogensis, Peter Bacchetti, Michael Roche, Carolin Tumpach, Matthew Gartner, Matthew C Pitman, Christine Lorrie Epling, Rebecca Hoh, Frederick M Hecht, Ma Somsouk, Paul U Cameron, Steven G Deeks, Sharon R Lewin

JOURNAL OF INFECTIOUS DISEASES | OXFORD UNIV PRESS INC | Published : 2020

Abstract

BACKGROUND: Identifying where human immunodeficiency virus (HIV) persists in people living with HIV and receiving antiretroviral therapy is critical to develop cure strategies. We assessed the relationship of HIV persistence to expression of chemokine receptors and their chemokines in blood (n = 48) and in rectal (n = 20) and lymph node (LN; n = 8) tissue collected from people living with HIV who were receiving suppressive antiretroviral therapy. METHODS: Cell-associated integrated HIV DNA, unspliced HIV RNA, and chemokine messenger RNA were quantified by quantitative polymerase chain reaction. Chemokine receptor expression on CD4+ T cells was determined using flow cytometry. RESULTS: Integr..

View full abstract

Grants

Awarded by National Institutes of Health Delaney AIDS Research Enterprise Collaboratory


Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

This work was supported by the National Institutes of Health Delaney AIDS Research Enterprise Collaboratory (grants U19 A1096109 and UM1AI126611), the National Health and Medical Research Council of Australia (program grant and practitioner fellowship to S. R. L. and postgraduate scholarships 579719 to G. K. and 1114265 to M. C. P.), and the Royal Australasian College of Physicians (CRB Blackburn Scholarship to M. C. P.).