Journal article

A GDSL Esterase/Lipase Catalyzes the Esterification of Lutein in Bread Wheat

Jacinta L Watkins, Ming Li, Ryan P McQuinn, Kai Xun Chan, Heather E McFarlane, Maria Ermakova, Robert T Furbank, Daryl Mares, Chongmei Dong, Kenneth J Chalmers, Peter Sharp, Diane E Mather, Barry J Pogson

The Plant Cell | OXFORD UNIV PRESS INC | Published : 2019

Abstract

Xanthophylls are a class of carotenoids that are important micronutrients for humans. They are often found esterified with fatty acids in fruits, vegetables, and certain grains, including bread wheat (Triticum aestivum). Esterification promotes the sequestration and accumulation of carotenoids, thereby enhancing stability, particularly in tissues such as in harvested wheat grain. Here, we report on a plant xanthophyll acyltransferase (XAT) that is both necessary and sufficient for xanthophyll esterification in bread wheat grain. XAT contains a canonical Gly-Asp-Ser-Leu (GDSL) motif and is encoded by a member of the GDSL esterase/lipase gene family. Genetic evidence from allelic variants of w..

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University of Melbourne Researchers

Grants

Awarded by Grains Research and Development Corporation


Awarded by Australian Research Council (ARC) Centre of Excellence in Plant Energy Biology


Awarded by ARC Centre of Excellence in Translational Photosynthesis


Awarded by ARC Discovery Early Career Reasearch Award


Awarded by Research Foundation - Flanders


Funding Acknowledgements

We thank Riya Kuruvilla for help with rice transformation and tissue culture; Robert Asenstorfer for assistance in lipid partitioning; Ian Dundas for providing seed of Langdon and the Langdon disomic substitution lines; Ursula Langridge for providing developing grains of Gladius; Gwen Mayo and Adelaide Microscopy, an AMMF facility at the University of Adelaide, for microscopy advice and services; and the International Wheat Genome SequencingConsortium (http://www.wheatgenome.org) for providing prepublication access to IWGSC RefSeq v1.0. Live cell imaging was conducted using equipment from the University of Melbourne Advanced Microscopy Facility and the Biological Optical Microscopy Platform. This research was funded by the Grains Research and Development Corporation (UA00102), Australian Research Council (ARC) Centre of Excellence in Plant Energy Biology (CE140100008), and the ARC Centre of Excellence in Translational Photosynthesis (CE1401000015). Support was also provided by ARC Discovery Early Career Reasearch Award (DE170100054 to H.E.M.), by the Research Foundation - Flanders (postdoctoral fellowship 12N4818N to K.X.C.), and by an Australian Research Training Program (RTP) scholarship to J.L.W.