Influenza A Virus-Infected Lung Epithelial Cell Co-Culture with Human Peripheral Blood Mononuclear Cells
Liyen Loh, Marios Koutsakos, Katherine Kedzierska, Timothy SC Hinks, H Kaipe (ed.), I Magalhaes (ed.)
MAIT CELLS: METHODS AND PROTOCOLS | HUMANA PRESS INC | Published : 2020
Sensing of influenza A virus (IAV) infection by pattern recognition receptors can occur by either direct infection of lung epithelial cells or uptake of virus-infected cells by innate cells such as dendritic cells/monocytes. This triggers a series of downstream events including activation of the inflammasome, the production of cytokines, chemokines, and the upregulation of stress-induced ligands that can lead to the activation of innate cells. These cells include innate lymphocytes such as MAIT, NKT, NK, and γδ T cells. Here we describe a method used to allow activation of human innate lymphocytes in co-culture with an IAV-infected human lung epithelial cell line (A549) to measure ex vivo ef..View full abstract
Related Projects (2)
Awarded by Wellcome Trust
Awarded by National Health and Medical Research Council (NHMRC)
This work was funded by grants to TSCH from the Wellcome Trust (104553/z/14/z, 211050/Z/18/z) and K.K from the National Health and Medical Research Council (NHMRC) Program Grant (1071916). K.K. is an NHMRC Senior Research Fellow (1102792). The content represents only the authors' views and not those of the European Commission.