Journal article

Influenza A Virus-Infected Lung Epithelial Cell Co-Culture with Human Peripheral Blood Mononuclear Cells

Liyen Loh, Marios Koutsakos, Katherine Kedzierska, Timothy SC Hinks, H Kaipe (ed.), I Magalhaes (ed.)

MAIT CELLS: METHODS AND PROTOCOLS | HUMANA PRESS INC | Published : 2020

Abstract

Sensing of influenza A virus (IAV) infection by pattern recognition receptors can occur by either direct infection of lung epithelial cells or uptake of virus-infected cells by innate cells such as dendritic cells/monocytes. This triggers a series of downstream events including activation of the inflammasome, the production of cytokines, chemokines, and the upregulation of stress-induced ligands that can lead to the activation of innate cells. These cells include innate lymphocytes such as MAIT, NKT, NK, and γδ T cells. Here we describe a method used to allow activation of human innate lymphocytes in co-culture with an IAV-infected human lung epithelial cell line (A549) to measure ex vivo ef..

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