CXCR4-Using HIV Strains Predominate in Naive and Central Memory CD4( ) T Cells in People Living with HIV on Antiretroviral Therapy: Implications for How Latency Is Established and Maintained
Michael Roche, Carolin Tumpach, Jori Symons, Matthew Gartner, Jenny L Anderson, Gabriela Khoury, Kieran Cashin, Paul U Cameron, Melissa J Churchill, Steven G Deeks, Paul R Gorry, Sharon R Lewin
JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2020
HIV can persist in people living with HIV (PLWH) on antiretroviral therapy (ART) in multiple CD4+ T cell subsets, including naive cells, central memory (CM) cells, transitional (TM) cells, and effector memory (EM) cells. Since these cells express different levels of the viral coreceptors CXCR4 and CCR5 on their surface, we sought to determine whether the HIV envelope protein (Env) was genotypically and phenotypically different between CD4+ T cell subsets isolated from PLWH on suppressive ART (n = 8). Single genome amplification for the HIV env gene was performed on genomic DNA extracts from different CD4+ T cell subsets. We detected CXCR4-using (X4) strains in five of the eight participants ..View full abstract
S.G.D. received grant support from Gilead, Merck, and ViiV, has consulted for Janssen and Abbvie, and is on the scientific advisory boards for Enochian Biosciences and Bryologix. S.R.L. has received investigator-initiated grant funding from Gilead, Merck, and ViiV. She has provided paid scientific advice to Abivax, Abbvie, and Gilead. The other authors declare no conflicts of interest.