Journal article

Mining the Methylome Reveals Extensive Diversity in Staphylococcus epidermidis Restriction Modification

Jean YH Lee, Glen P Carter, Sacha J Pidot, Romain Guerillot, Torsten Seemann, Anders Goncalves da Silva, Timothy J Foster, Benjamin P Howden, Timothy P Stinear, Ian R Monk

MBIO | AMER SOC MICROBIOLOGY | Published : 2019

Abstract

Staphylococcus epidermidis is a significant opportunistic pathogen of humans. Molecular studies in this species have been hampered by the presence of restriction-modification (RM) systems that limit introduction of foreign DNA. Here, we establish the complete genomes and methylomes for seven clinically significant, genetically diverse S. epidermidis isolates and perform the first systematic genomic analyses of the type I RM systems within both S. epidermidis and Staphylococcus aureus Our analyses revealed marked differences in the gene arrangement, chromosomal location, and movement of type I RM systems between the two species. Unlike S. aureus, S. epidermidis type I RM systems demonstrate e..

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Grants

Awarded by National Health and Medical Research Council of Australia (NHMRC) Senior Research Fellowship


Awarded by NHMRC


Funding Acknowledgements

This project was supported by the Royal Australasian College of Physicians, Basser Research Entry Scholarship/Australian Government Research Training Program Scholarship (to J.Y.H.L.); a National Health and Medical Research Council of Australia (NHMRC) Senior Research Fellowship (to T.P.S.) (GNT1105525); an NHMRC Practitioner Fellowship to B.P.H. (GNT1105905); and an NHMRC Project Grant (GNT1066791).