Journal article
Replacing murine insulin 1 with human insulin protects NOD mice from diabetes
CM Elso, NA Scott, L Mariana, EI Masterman, APR Sutherland, HE Thomas, SI Mannering
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2019
Abstract
Type 1, or autoimmune, diabetes is caused by the T-cell mediated destruction of the insulin-producing pancreatic beta cells. Non-obese diabetic (NOD) mice spontaneously develop autoimmune diabetes akin to human type 1 diabetes. For this reason, the NOD mouse has been the preeminent murine model for human type 1 diabetes research for several decades. However, humanized mouse models are highly sought after because they offer both the experimental tractability of a mouse model and the clinical relevance of human-based research. Autoimmune T-cell responses against insulin, and its precursor proinsulin, play central roles in the autoimmune responses against pancreatic beta cells in both humans an..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by a: A Millennium Award (Y17M1-MANS) from Diabetes Australia (SM) and a Diabetes Australia Project grant to CE (Y18G-ELSC); Australian National Health and Medical Research Council (GNT1123586) to SM and JDRF (JDRF 2-SRA-2018-568-S-B) to SM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.The mutant mice were produced via CRISPR/Cas9 oocyte injection by Monash University as a node of the Australian Phenomics Network (APN). The APN is supported by the Australian Government Department of Education through the National Collaborative Research Infrastructure Scheme.