Visualizing the Selectivity and Dynamics of Interferon Signaling In Vivo
Sebastian A Stifter, Nayan Bhattacharyya, Andrew J Sawyer, Taylor A Cootes, John Stambas, Sean E Doyle, Lionel Feigenbaum, William E Paul, Warwick J Britton, Alan Sher, Carl G Feng
CELL REPORTS | CELL PRESS | Published : 2019
Interferons (IFN) are pleiotropic cytokines essential for defense against infection, but the identity and tissue distribution of IFN-responsive cells in vivo are poorly defined. In this study, we generate a mouse strain capable of reporting IFN-signaling activated by all three types of IFNs and investigate the spatio-temporal dynamics and identity of IFN-responding cells following IFN injection and influenza virus infection. Despite ubiquitous expression of IFN receptors, cellular responses to IFNs are highly heterogenous in vivo and are determined by anatomical site, cell type, cellular preference to individual IFNs, and activation status. Unexpectedly, type I and II pneumocytes, the primar..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) of Australia
We thank C. Watson and J. Zhu for their advice in constructing the M1Red reporter mouse strain and B. Roediger and M. Coleman for critical reading of this manuscript. We acknowledge the Centenary Institute animal and flow cytometry core facilities for their contributions to this work. This work was supported by a National Health and Medical Research Council (NHMRC) of Australia Project (APP1051742). N.B., A.J.S., and T.A.C are supported by Australian Postgraduate Awards.