The developmental phosphoproteome of Haemonchus contortus
Tao Wang, Guangxu Ma, Ching-Seng Ang, Pasi K Korhonen, Andreas J Stroehlein, Neil D Young, Andreas Hofmann, Bill CH Chang, Nicholas A Williamson, Robin B Gasser
JOURNAL OF PROTEOMICS | ELSEVIER | Published : 2020
Protein phosphorylation plays essential roles in many cellular processes. Despite recent progress in the genomics, transcriptomics and proteomics of socioeconomically important parasitic nematodes, there is scant phosphoproteomic data to underpin molecular biological discovery. Here, using the phosphopeptide enrichment-based LC-MS/MS and data-independent acquisition (DIA) quantitation, we characterised the first developmental phosphoproteome of the parasitic nematode Haemonchus contortus - one of the most pathogenic parasites of ruminant livestock. Totally, 1804 phosphorylated proteins with 4406 phosphorylation sites ('phosphosites') from different developmental stages/sexes were identified...View full abstract
Support from the National Health and Medical Research Council (NHMRC) of Australia, the Australian Research Council, Yourgene Health, Melbourne Water Corporation and The University of Melbourne (BIP) is gratefully acknowledged (R.B.G. et al.). P.K.K. holds an NHMRC Early Career Researcher Fellowship (ECRF). N.D.Y. holds an NHMRC Career Development Fellowship (CDF1). Funding bodies played no role in the design of the study or the collection, analysis or interpretation of data, or the writing of the manuscript.