Journal article

A PCR assay to quantify patterns of HBV transcription

Valentina D'Arienzo, Andrea Magri, James M Harris, Peter AC Wing, Chunkyu Ko, Claudia Orbegozo Rubio, Peter A Revill, Ulrike Protzer, Peter Balfe, Jane A McKeating

JOURNAL OF GENERAL VIROLOGY | MICROBIOLOGY SOC | Published : 2021

Abstract

Hepatitis B virus (HBV) is the prototype member of the family Hepadnaviridae and replicates via episomal copies of a covalently closed circular DNA (cccDNA) genome of approximately 3.2 kb. The chromatinization of this small viral genome, with overlapping open reading frames and regulatory elements, suggests an important role for epigenetic pathways to regulate HBV transcription. However, the host pathways that regulate HBV transcription and the temporal nature of promoter usage in infected cells are not well understood, in part due to the compact genome structure and overlapping open reading frames. To address this we developed a simple and cost-effective PCR assay to quantify the major vira..

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University of Melbourne Researchers

Grants

Awarded by EU 2020 Research and Innovation Programme Consortia HEP-CAR


Awarded by MRC


Awarded by Wellcome Trust


Funding Acknowledgements

This work was supported by EU 2020 Research and Innovation Programme Consortia HEP-CAR under grant agreement no. 667273, MRC MR/R022011/1 and Wellcome Trust IA 200838/Z/16/Z. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.