Journal article

The majority of A-to-I RNA editing is not required for mammalian homeostasis

Alistair M Chalk, Scott Taylor, Jacki E Heraud-Farlow, Carl R Walkley

Genome Biology | BMC | Published : 2019

Abstract

BACKGROUND: Adenosine-to-inosine (A-to-I) RNA editing, mediated by ADAR1 and ADAR2, occurs at tens of thousands to millions of sites across mammalian transcriptomes. A-to-I editing can change the protein coding potential of a transcript and alter RNA splicing, miRNA biology, RNA secondary structure and formation of other RNA species. In vivo, the editing-dependent protein recoding of GRIA2 is the essential function of ADAR2, while ADAR1 editing prevents innate immune sensing of endogenous RNAs by MDA5 in both human and mouse. However, a significant proportion of A-to-I editing sites can be edited by both ADAR1 and ADAR2, particularly within the brain where both are highly expressed. The phys..

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Grants

Awarded by National Health and Medical Research Council, Australia (NHMRC)


Awarded by Australian Research Council


Awarded by Victorian Cancer Agency Research Fellowship


Funding Acknowledgements

This work was supported by the National Health and Medical Research Council, Australia (NHMRC; CRW and JB Li, APP1102006/APP1144049); Australian Research Council (CRW; DP180103989); Victorian Cancer Agency Research Fellowship (CRW; MCRF15015); Marian and E.H. Flack Trust (JH-F); SVI Women in Science Award (JH-F); and in part by the Victorian State Government Operational Infrastructure Support (to St Vincent's Institute).