Journal article

Limitations of dual-fluorescent HIV reporter viruses in a model of pre-activation latency

Youry Kim, Paul U Cameron, Sharon R Lewin, Jenny L Anderson

Journal of the International AIDS Society | JOHN WILEY & SONS LTD | Published : 2019

Abstract

INTRODUCTION: HIV latency can be established in vitro following direct infection of a resting CD4+ T cell (pre-activation latency) or infection of an activated CD4+ T cell which then returns to a resting state (post-activation latency). We modified a previously published dual-fluorescent reporter virus seeking to track the establishment and reactivation of pre-activation latency in primary CD4+ T cells. METHODS: A previously published dual-fluorescent reporter virus was modified so that expression of enhanced green fluorescent protein (GFP) was under control of the elongation factor 1 alpha (EF1α) promoter to detect latent infection, and E2 crimson (E2CRM) was under control of the nef promot..

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