Limitations of dual-fluorescent HIV reporter viruses in a model of pre-activation latency
Youry Kim, Paul U Cameron, Sharon R Lewin, Jenny L Anderson
JOURNAL OF THE INTERNATIONAL AIDS SOCIETY | JOHN WILEY & SONS LTD | Published : 2019
INTRODUCTION: HIV latency can be established in vitro following direct infection of a resting CD4+ T cell (pre-activation latency) or infection of an activated CD4+ T cell which then returns to a resting state (post-activation latency). We modified a previously published dual-fluorescent reporter virus seeking to track the establishment and reactivation of pre-activation latency in primary CD4+ T cells. METHODS: A previously published dual-fluorescent reporter virus was modified so that expression of enhanced green fluorescent protein (GFP) was under control of the elongation factor 1 alpha (EF1α) promoter to detect latent infection, and E2 crimson (E2CRM) was under control of the nef promot..View full abstract
This work was funded by the National Health and Medical Research Council of Australia (SRL).