Journal article

A Molecular Chameleon for Mapping Subcellular Polarity in an Unfolded Proteome Environment

Tze Cin Owyong, Pramod Subedi, Jieru Deng, Elizabeth Hinde, Jason J Paxman, Jonathan M White, Weisan Chen, Begona Heras, Wallace WH Wong, Yuning Hong

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | WILEY-V C H VERLAG GMBH | Published : 2020

Abstract

Environmental polarity is an important factor that drives biomolecular interactions to regulate cell function. Herein, a general method of using the fluorogenic probe NTPAN-MI is reported to quantify the subcellular polarity change in response to protein unfolding. NTPAN-MI fluorescence is selectively activated upon labeling unfolded proteins with exposed thiols, thereby reporting on the extent of proteostasis. NTPAN-MI also reveals the collapse of the host proteome caused by influenza A virus infection. The emission profile of NTPAN-MI contains information of the local polarity of the unfolded proteome, which can be resolved through spectral phasor analysis. Under stress conditions that dis..

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