Targeted viral vector transduction of relaxin-3 neurons in the rat nucleus incertus using a novel cell-type specific promoter
Alexander D Wykes, Sherie Ma, Ross AD Bathgate, Andrew L Gundlach
IBRO REPORTS | ELSEVIER | Published : 2020
Modern neuroscience utilizes transgenic techniques extensively to study the activity and function of brain neural networks. A key feature of this approach is its compatibility with molecular methods for selective transgene expression in neuronal circuits of interest. Until now, such targeted transgenic approaches have not been applied to the extensive circuitry involving the neuropeptide, relaxin-3. Pharmacological and gene knock-out studies have revealed relaxin-3 signalling modulates interrelated behaviours and cognitive processes, including stress and anxiety, food and alcohol consumption, and spatial and social memory, highlighting the potential of this system as a therapeutic target. In..View full abstract
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Awarded by National Health and Medical Research Council (NHMRC) of Australia
This research was supported by a National Health and Medical Research Council (NHMRC) of Australia project grant (1067522, ALG, RADB), a generous, private Florey donor (ADW, ALG), and the Victorian Government Operational Infrastructure Support Programme (The Florey Institute of Neuroscience and Mental Health). ADW is the recipient of an Alzheimer's Australia Dementia Research Foundation Postgraduate Scholarship, and RADB is the recipient of an NHMRC (Australia) Senior Research Fellowship. The authors thank Sharon Layfield (The Florey Institute of Neuroscience and Mental Health) for assistance in isolating the described relaxin-3 promoter sequence from rat genomic DNA.