A potential association between IL-3 and type I and III interferons in systemic lupus erythematosus
Shereen Oon, Katherine Monaghan, Milica Ng, Alberta Hoi, Eric Morand, Gino Vairo, Eugene Maraskovsky, Andrew D Nash, Ian P Wicks, Nicholas J Wilson
Clinical and Translational Immunology | WILEY | Published : 2019
Objectives: Plasmacytoid dendritic cells (pDCs), through the production of type 1 interferons (IFNs) and other cytokines, are major contributors to systemic lupus erythematosus (SLE) pathogenesis. IL-3 promotes pDC survival, but its role in SLE is not well characterised. This study investigated serum IL-3 and IFN levels, and a whole blood 'IL-3 gene signature', in human SLE. Methods: Serum cytokine levels were measured by ELISA in n = 42 SLE patients, and n = 44 healthy donors. IL-3-regulated genes were determined by RNASeq of healthy donor whole blood cells (WBCs) stimulated in vitro with IL-3 for 6 or 24 h. Whole blood cell RNASeq analysis was undertaken in a separate cohort of n = 31 SLE ..View full abstract
Awarded by NHMRC Australia
This work was supported by the Reid Charitable Trusts, NHMRC Australia (IPW Clinical Practitioner Fellowship (1023407) and NHMRC Program Grant (1016647), SO Postgraduate Scholarship (1039026)), operational infrastructure grants through the Australian Government Institute for Research and Innovation in Social Services and the Victorian State Government, Arthritis Australia (South Australia Lupus, Scleroderma and Sjogren's Support Group Grant), CSL Limited and Janssen Research and Development, LLC.