Rapid PCR identification of Prevotella copri in an Australian cohort of pregnant women.
Lawrence Gray, Kyoko Hasebe, Martin O'Hely, Anne-Louise Ponsonby, Peter Vuillermin, Fiona Collier, undefined BIS Investigator Group
Journal of Developmental Origins of Health and Disease | Published : 2020
Gut bacteria from the genus Prevotella are found in high abundance in faeces of non-industrialised communities but low abundance in industrialised, Westernised communities. Prevotella copri is one of the principal Prevotella species within the human gut. As it has been associated with developmental health and disease states, we sought to (i) develop a real-time polymerase chain reaction (PCR) to rapidly determine P. copri abundance and (ii) investigate its abundance in a large group of Australian pregnant mothers.The Barwon Infant Study is a pre-birth cohort study (n = 1074). Faecal samples were collected from mothers at 36 weeks gestation. Primers with a probe specific to the V3 region of P..View full abstract