Latest techniques to study DNA methylation
Quentin Gouil, Andrew Keniry, M Blewitt (ed.)
DNA METHYLATION | PORTLAND PRESS LTD | Published : 2019
Bisulfite sequencing is a powerful technique to detect 5-methylcytosine in DNA that has immensely contributed to our understanding of epigenetic regulation in plants and animals. Meanwhile, research on other base modifications, including 6-methyladenine and 4-methylcytosine that are frequent in prokaryotes, has been impeded by the lack of a comparable technique. Bisulfite sequencing also suffers from a number of drawbacks that are difficult to surmount, among which DNA degradation, lack of specificity, or short reads with low sequence diversity. In this review, we explore the recent refinements to bisulfite sequencing protocols that enable targeting genomic regions of interest, detecting der..View full abstract
Awarded by National Health and Medical Research Council
A.K. receives funding from the National Health and Medical Research Council [grant number 1140976].