Two isoprenylated flavonoids from Dorstenia psilurus activate AMPK, stimulate glucose uptake, inhibit glucose production and lower glycemia.
Aphrodite T Choumessi, Manuel Johanns, Claire Beaufay, Marie-France Herent, Vincent Stroobant, Didier Vertommen, Cyril Corbet, Roxane Jacobs, Gaëtan Herinckx, Gregory R Steinberg, Olivier Feron, Joëlle Quetin-Leclercq, Mark H Rider
Biochem J | Published : 2019
Root extracts of a Cameroon medicinal plant, Dorstenia psilurus, were purified by screening for AMP-activated protein kinase (AMPK) activation in incubated mouse embryo fibroblasts (MEFs). Two isoprenylated flavones that activated AMPK were isolated. Compound 1 was identified as artelasticin by high-resolution electrospray ionization mass spectrometry and 2D-NMR while its structural isomer, compound 2, was isolated for the first time and differed only by the position of one double bond on one isoprenyl substituent. Treatment of MEFs with purified compound 1 or compound 2 led to rapid and robust AMPK activation at low micromolar concentrations and increased the intracellular AMP:ATP ratio. In..View full abstract