Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS.
Debnath Ghosal, Kwangcheol C Jeong, Yi-Wei Chang, Jacob Gyore, Lin Teng, Adam Gardner, Joseph P Vogel, Grant J Jensen
Nature Microbiology | Published : 2019
Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). Here, we used complementary electron cryotomography and immunofluorescence microscopy to investigate the molecular architecture and biogenesis of the Dot/Icm secretion apparatus. Electron cryotomography mapped the location of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a well-ordered central channel that opens into a large, windowed secretion chamber with an unusual 13-fold symmetry. Immunofluorescence microscopy deciphered an earl..View full abstract