Journal article

Development of a Novel CD4 TCR Transgenic Line that Reveals a Dominant Role for CD8 DC and CD40-Signaling in the Generation of Helper and CTL Responses to Blood Stage Malaria

Daniel Fernandez-Ruiz, Lei Shong Lau, Nazanin Ghazanfari, Claerwen Jones, Wei Yi Ng, Gayle Davey, Dorothee Berthold, Lauren Holz, Yu Kato, Ganchimeg Bayarsaikhan, Sanne Hendriks, Kylie James, Anton Cozijnsen, Vanessa Mollard, Tania de Koning-Ward, Paul Gilson, Tsuneyasu Kaisho, Ashraful Haque, Brendan Crabb, Francis Carbone Show all

Published : 2017

Abstract

Abstract We describe an MHC II (IA b )-restricted T cell receptor (TCR) transgenic mouse line that produces CD4 + T cells specific for Plasmodium species. This line, termed PbT-II, was derived from a CD4 + T cell hybridoma generated to blood-stage Plasmodium berghei ANKA (PbA). PbT-II cells responded to all Plasmodium species and stages tested so far, including rodent (PbA, P. berghei NK65, P. chabaudi AS and P. yoelii 17XNL) and human ( P . falciparum) blood-stage parasites as well as irradiated PbA sporozoites. PbT-II cells can provide help for generation of antibody to P. chabaudi infection and can control this otherwise lethal infection in CD40L-deficient mice. PbT-II cells can also prov..

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University of Melbourne Researchers

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