Journal article

Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley

Ravi Koppolu, Nadia Anwar, Shun Sakuma, Akemi Tagiri, Udda Lundqvist, Mohammad Pourkheirandish, Twan Rutten, Christiane Seiler, Axel Himmelbach, Ruvini Ariyadasa, Helmy Mohamad Youssef, Nils Stein, Nese Sreenivasulu, Takao Komatsuda, Thorsten Schnurbusch

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2013

Abstract

Inflorescence architecture of barley (Hordeum vulgare L.) is common among the Triticeae species, which bear one to three single-flowered spikelets at each rachis internode. Triple spikelet meristem is one of the unique features of barley spikes, in which three spikelets (one central and two lateral spikelets) are produced at each rachis internode. Fertility of the lateral spikelets at triple spikelet meristem gives row-type identity to barley spikes. Six-rowed spikes show fertile lateral spikelets and produce increased grain yield per spike, compared with two-rowed spikes with sterile lateral spikelets. Thus, far, two loci governing the row-type phenotype were isolated in barley that include..

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Grants

Awarded by Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics for Agricultural Innovation Grant)


Awarded by Deutsche Forschungsgemeinschaft (DFG Grant)


Awarded by BMBF (German Federal Ministry of Education and Research, GABI-FUTURE Start_Young Investigator Program Grant)


Funding Acknowledgements

We thank Dr. S. R. Palakolanu (International Crops Research Institute for the Semi-Arid Tropics) for help with phylogenetic analysis; Dr. B. Kilian (IPK Gatersleben) for providing germplasm for haplotype analysis; H. Bockelman (US Department of Agriculture-Agricultural Research Service) and IPK gene bank for providing initial mutant germplasm; I. Walde for help with the VeraCode experiment; P. Gawronski for helpful discussions; and H. Ernst, H. Koyama, S. Konig, K. Lipfert, M. Puffeld, M. Purschel, C. Trautewig, and C. Weissleder for excellent technical support. This work was supported by grants from the Ministry of Education (IZN), Saxony-Anhalt (to N. Sreenivasulu), the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics for Agricultural Innovation Grant TRG1004; to T.K.), the Deutsche Forschungsgemeinschaft (DFG Grant SCHN 768/2-1; to T.S.), and the BMBF (German Federal Ministry of Education and Research, GABI-FUTURE Start_Young Investigator Program Grant 0315071; to T.S.).