Journal article

miR172 downregulates the translation of cleistogamy 1 in barley

Nadia Anwar, Masaru Ohta, Takayuki Yazawa, Yutaka Sato, Chao Li, Akemi Tagiri, Mari Sakuma, Thomas Nussbaumer, Phil Bregitzer, Mohammad Pourkheirandish, Jianzhong Wu, Takao Komatsuda

Annals of Botany | Oxford University Press (OUP) | Published : 2018

Abstract

Background and Aims Floret opening in barley is induced by the swelling of the lodicule, a trait under the control of the cleistogamy1 (cly1) gene. The product of cly1 is a member of the APETALA2 (AP2) transcription factor family, which inhibits lodicule development. A sequence polymorphism at the miR172 target site within cly1 has been associated with variation in lodicule development and hence with the cleistogamous phenotype. It was unclear whether miR172 actually functions in cly1 regulation and, if it does, which miR172 gene contributes to cleistogamy. It was also interesting to explore whether miR172-mediated cly1 regulation occurs at transcriptional level or at translational level. M..

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Grants

Awarded by Japanese Ministry of Agriculture, Forestry and Fisheries Genomics for Agricultural Innovation programme


Funding Acknowledgements

We thank Ning Wang for help in antibody design, Shun Sakuma for help in qRT-PCR, Nils Stein for access to the barley genome sequences, Harumi Koyama for taking care of the plants and helping to sample the spikes, Hisae Kamakura for preparing material for laser microdissection, and the NIAS Genebank (Tsukuba, Japan) for the provision of seeds of the various barley cultivars. This research was supported by the Japanese Ministry of Agriculture, Forestry and Fisheries Genomics for Agricultural Innovation programme (TRS1001 to J.W. and TRS1002 and TRS1005 to T.K.).